Construct: shRNA TRCN0000049596

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376684.1-1311s1c1
DNA Barcode:
CCCTATAATAATGGAAGCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGAM2 (93432)
Gene Description:
maltase-glucoamylase 2 (putative)
Transcript:
RefSeq XM_376684.1 (NON-CURRENT)
Match location:
Position 1311 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049596)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATAATAATGGAAGCCTA
Hairpin Sequence:
5'-CCGG-CCCTATAATAATGGAAGCCTA-CTCGAG-TAGGCTTCCATTATTATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATAATAATGGAAGCCTACTCGAGTAGGCTTCCATTATTATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATAATAATGGAAGCCTACTCGAGTAGGCTTCCATTATTATAGGG-3'

Other clones with same target sequence:

(none)