Construct: shRNA TRCN0000049639

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020379.1-1541s1c1
DNA Barcode:
GCAAGACAGATATGGAGGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAN1C1 (57134)
Gene Description:
mannosidase alpha class 1C member 1
Transcript:
RefSeq NM_020379.1 (NON-CURRENT)
Match location:
Position 1541 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049639)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGACAGATATGGAGGCTA
Hairpin Sequence:
5'-CCGG-GCAAGACAGATATGGAGGCTA-CTCGAG-TAGCCTCCATATCTGTCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGACAGATATGGAGGCTACTCGAGTAGCCTCCATATCTGTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGACAGATATGGAGGCTACTCGAGTAGCCTCCATATCTGTCTTGC-3'

Other clones with same target sequence:

(none)