Construct: shRNA TRCN0000049799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002937.3-785s1c1
DNA Barcode:
CCATGAAGATATCTGGAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNASE4 (6038)
Gene Description:
ribonuclease A family member 4
Transcript:
RefSeq NM_002937.3 (NON-CURRENT)
Match location:
Position 785 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGAAGATATCTGGAACAT
Hairpin Sequence:
5'-CCGG-CCATGAAGATATCTGGAACAT-CTCGAG-ATGTTCCAGATATCTTCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGAAGATATCTGGAACATCTCGAGATGTTCCAGATATCTTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGAAGATATCTGGAACATCTCGAGATGTTCCAGATATCTTCATGG-3'

Other clones with same target sequence:

  1. TRCN0000291904