Construct: shRNA TRCN0000049806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175854.4-690s1c1
DNA Barcode:
CGTTGCTTATATGCAACCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAN3 (255967)
Gene Description:
poly(A) specific ribonuclease subunit PAN3
Transcript:
RefSeq NM_175854.4 (NON-CURRENT)
Match location:
Position 690 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGCTTATATGCAACCGAA
Hairpin Sequence:
5'-CCGG-CGTTGCTTATATGCAACCGAA-CTCGAG-TTCGGTTGCATATAAGCAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGCTTATATGCAACCGAACTCGAGTTCGGTTGCATATAAGCAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGCTTATATGCAACCGAACTCGAGTTCGGTTGCATATAAGCAACG-3'

Other clones with same target sequence:

(none)