Construct: shRNA TRCN0000049851

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003730.3-579s1c1
DNA Barcode:
CCTCCGGATTACTGGACAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNASET2 (8635)
Gene Description:
ribonuclease T2
Transcript:
RefSeq NM_003730.3 (NON-CURRENT)
Match location:
Position 579 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049851)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCGGATTACTGGACAATA
Hairpin Sequence:
5'-CCGG-CCTCCGGATTACTGGACAATA-CTCGAG-TATTGTCCAGTAATCCGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCGGATTACTGGACAATACTCGAGTATTGTCCAGTAATCCGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCGGATTACTGGACAATACTCGAGTATTGTCCAGTAATCCGGAGG-3'

Other clones with same target sequence:

(none)