Construct: shRNA TRCN0000049916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032043.1-444s1c1
DNA Barcode:
CCAAGGAACTTCACGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRIP1 (83990)
Gene Description:
BRCA1 interacting protein C-terminal helicase 1
Transcript:
RefSeq NM_032043.1 (NON-CURRENT)
Match location:
Position 444 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGAACTTCACGTCATTT
Hairpin Sequence:
5'-CCGG-CCAAGGAACTTCACGTCATTT-CTCGAG-AAATGACGTGAAGTTCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGAACTTCACGTCATTTCTCGAGAAATGACGTGAAGTTCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGAACTTCACGTCATTTCTCGAGAAATGACGTGAAGTTCCTTGG-3'

Other clones with same target sequence:

(none)