Construct: shRNA TRCN0000049924

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019598.2-594s1c1
DNA Barcode:
CCAGTGCCTCAACCTCTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLK12 (43849)
Gene Description:
kallikrein related peptidase 12
Transcript:
RefSeq NM_019598.2 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049924)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGCCTCAACCTCTCCAT
Hairpin Sequence:
5'-CCGG-CCAGTGCCTCAACCTCTCCAT-CTCGAG-ATGGAGAGGTTGAGGCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGCCTCAACCTCTCCATCTCGAGATGGAGAGGTTGAGGCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGCCTCAACCTCTCCATCTCGAGATGGAGAGGTTGAGGCACTGG-3'

Other clones with same target sequence:

(none)