Construct: shRNA TRCN0000049960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145699.2-105s1c1
DNA Barcode:
CACTTGATGGATCCACACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APOBEC3A (200315)
Gene Description:
apolipoprotein B mRNA editing enzyme catalytic subunit 3A
Transcript:
RefSeq NM_145699.2 (NON-CURRENT)
Match location:
Position 105 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTGATGGATCCACACATA
Hairpin Sequence:
5'-CCGG-CACTTGATGGATCCACACATA-CTCGAG-TATGTGTGGATCCATCAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTGATGGATCCACACATACTCGAGTATGTGTGGATCCATCAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTGATGGATCCACACATACTCGAGTATGTGTGGATCCATCAAGTG-3'

Other clones with same target sequence:

(none)