Construct: shRNA TRCN0000049971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003895.2-2309s1c1
DNA Barcode:
GCAGGAGATCAACTTATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNJ1 (8867)
Gene Description:
synaptojanin 1
Transcript:
RefSeq NM_003895.2 (NON-CURRENT)
Match location:
Position 2309 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAGATCAACTTATCAAT
Hairpin Sequence:
5'-CCGG-GCAGGAGATCAACTTATCAAT-CTCGAG-ATTGATAAGTTGATCTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAGATCAACTTATCAATCTCGAGATTGATAAGTTGATCTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAGATCAACTTATCAATCTCGAGATTGATAAGTTGATCTCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000288984