Construct: shRNA TRCN0000049982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020963.1-2591s1c1
DNA Barcode:
CGTTACTGCATCACCAAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MOV10 (4343)
Gene Description:
Mov10 RISC complex RNA helicase
Transcript:
RefSeq NM_020963.1 (NON-CURRENT)
Match location:
Position 2591 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTACTGCATCACCAAACTT
Hairpin Sequence:
5'-CCGG-CGTTACTGCATCACCAAACTT-CTCGAG-AAGTTTGGTGATGCAGTAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTACTGCATCACCAAACTTCTCGAGAAGTTTGGTGATGCAGTAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTACTGCATCACCAAACTTCTCGAGAAGTTTGGTGATGCAGTAACG-3'

Other clones with same target sequence:

(none)