Construct: shRNA TRCN0000049995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022126.2-338s1c1
DNA Barcode:
CCGCTCAGAATTTGATCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LHPP (64077)
Gene Description:
phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Transcript:
RefSeq NM_022126.2 (NON-CURRENT)
Match location:
Position 338 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTCAGAATTTGATCAGAT
Hairpin Sequence:
5'-CCGG-CCGCTCAGAATTTGATCAGAT-CTCGAG-ATCTGATCAAATTCTGAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTCAGAATTTGATCAGATCTCGAGATCTGATCAAATTCTGAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTCAGAATTTGATCAGATCTCGAGATCTGATCAAATTCTGAGCGG-3'

Other clones with same target sequence:

  1. TRCN0000333134