Construct: shRNA TRCN0000050143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152624.3-1267s1c1
DNA Barcode:
CCACGGAAACTTCAGGATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCP2 (167227)
Gene Description:
decapping mRNA 2
Transcript:
RefSeq NM_152624.3 (NON-CURRENT)
Match location:
Position 1267 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGGAAACTTCAGGATAAT
Hairpin Sequence:
5'-CCGG-CCACGGAAACTTCAGGATAAT-CTCGAG-ATTATCCTGAAGTTTCCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGGAAACTTCAGGATAATCTCGAGATTATCCTGAAGTTTCCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGGAAACTTCAGGATAATCTCGAGATTATCCTGAAGTTTCCGTGG-3'

Other clones with same target sequence:

  1. TRCN0000288954