Construct: shRNA TRCN0000050160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130901.1-1538s1c1
DNA Barcode:
GCAGCAATTCTAACAGCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OTUD7A (161725)
Gene Description:
OTU deubiquitinase 7A
Transcript:
RefSeq NM_130901.1 (NON-CURRENT)
Match location:
Position 1538 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAATTCTAACAGCAATA
Hairpin Sequence:
5'-CCGG-GCAGCAATTCTAACAGCAATA-CTCGAG-TATTGCTGTTAGAATTGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAATTCTAACAGCAATACTCGAGTATTGCTGTTAGAATTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAATTCTAACAGCAATACTCGAGTATTGCTGTTAGAATTGCTGC-3'

Other clones with same target sequence:

(none)