Construct: shRNA TRCN0000050197

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030791.2-878s1c1
DNA Barcode:
GCTGGATTCCTATATACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGPP1 (81537)
Gene Description:
sphingosine-1-phosphate phosphatase 1
Transcript:
RefSeq NM_030791.2 (NON-CURRENT)
Match location:
Position 878 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050197)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATTCCTATATACCATT
Hairpin Sequence:
5'-CCGG-GCTGGATTCCTATATACCATT-CTCGAG-AATGGTATATAGGAATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATTCCTATATACCATTCTCGAGAATGGTATATAGGAATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATTCCTATATACCATTCTCGAGAATGGTATATAGGAATCCAGC-3'

Other clones with same target sequence:

(none)