Construct: shRNA TRCN0000050206

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017829.4-214s1c1
DNA Barcode:
TGTTACAAATGCTGGGAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDHD5 (27440)
Gene Description:
haloacid dehalogenase like hydrolase domain containing 5
Transcript:
RefSeq NM_017829.4 (NON-CURRENT)
Match location:
Position 214 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050206)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTACAAATGCTGGGAACAT
Hairpin Sequence:
5'-CCGG-TGTTACAAATGCTGGGAACAT-CTCGAG-ATGTTCCCAGCATTTGTAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTACAAATGCTGGGAACATCTCGAGATGTTCCCAGCATTTGTAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTACAAATGCTGGGAACATCTCGAGATGTTCCCAGCATTTGTAACA-3'

Other clones with same target sequence:

(none)