Construct: shRNA TRCN0000050231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000204.1-337s1c1
DNA Barcode:
CCGAAGGAAAGTTTAGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFI (3426)
Gene Description:
complement factor I
Transcript:
RefSeq NM_000204.1 (NON-CURRENT)
Match location:
Position 337 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAGGAAAGTTTAGTGTTT
Hairpin Sequence:
5'-CCGG-CCGAAGGAAAGTTTAGTGTTT-CTCGAG-AAACACTAAACTTTCCTTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAGGAAAGTTTAGTGTTTCTCGAGAAACACTAAACTTTCCTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAGGAAAGTTTAGTGTTTCTCGAGAAACACTAAACTTTCCTTCGG-3'

Other clones with same target sequence:

(none)