Construct: shRNA TRCN0000050243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000036.1-567s1c1
DNA Barcode:
CCCTTCCAAATACTTGCGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMPD1 (270)
Gene Description:
adenosine monophosphate deaminase 1
Transcript:
RefSeq NM_000036.1 (NON-CURRENT)
Match location:
Position 567 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCCAAATACTTGCGGAA
Hairpin Sequence:
5'-CCGG-CCCTTCCAAATACTTGCGGAA-CTCGAG-TTCCGCAAGTATTTGGAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCCAAATACTTGCGGAACTCGAGTTCCGCAAGTATTTGGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCCAAATACTTGCGGAACTCGAGTTCCGCAAGTATTTGGAAGGG-3'

Other clones with same target sequence:

(none)