Construct: shRNA TRCN0000050406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022089.1-298s1c1
DNA Barcode:
CGTTATCGAAATAAGAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP13A2 (23400)
Gene Description:
ATPase cation transporting 13A2
Transcript:
RefSeq NM_022089.1 (NON-CURRENT)
Match location:
Position 298 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTATCGAAATAAGAGACAA
Hairpin Sequence:
5'-CCGG-CGTTATCGAAATAAGAGACAA-CTCGAG-TTGTCTCTTATTTCGATAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTATCGAAATAAGAGACAACTCGAGTTGTCTCTTATTTCGATAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTATCGAAATAAGAGACAACTCGAGTTGTCTCTTATTTCGATAACG-3'

Other clones with same target sequence:

(none)