Construct: shRNA TRCN0000050535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006773.3-933s1c1
DNA Barcode:
CGTGCATACCTATGGCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX18 (8886)
Gene Description:
DEAD-box helicase 18
Transcript:
RefSeq NM_006773.3 (NON-CURRENT)
Match location:
Position 933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCATACCTATGGCTTGAT
Hairpin Sequence:
5'-CCGG-CGTGCATACCTATGGCTTGAT-CTCGAG-ATCAAGCCATAGGTATGCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCATACCTATGGCTTGATCTCGAGATCAAGCCATAGGTATGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCATACCTATGGCTTGATCTCGAGATCAAGCCATAGGTATGCACG-3'

Other clones with same target sequence:

  1. TRCN0000290665