Construct: shRNA TRCN0000050538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001585.2-980s1c1
DNA Barcode:
CCTATGTGAATGCGTCCGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPPED1 (758)
Gene Description:
metallophosphoesterase domain containing 1
Transcript:
RefSeq NM_001585.2 (NON-CURRENT)
Match location:
Position 980 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATGTGAATGCGTCCGTAT
Hairpin Sequence:
5'-CCGG-CCTATGTGAATGCGTCCGTAT-CTCGAG-ATACGGACGCATTCACATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATGTGAATGCGTCCGTATCTCGAGATACGGACGCATTCACATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATGTGAATGCGTCCGTATCTCGAGATACGGACGCATTCACATAGG-3'

Other clones with same target sequence:

(none)