Construct: shRNA TRCN0000050541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001585.2-568s1c1
DNA Barcode:
GACCTCATCAAGCAGGACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPPED1 (758)
Gene Description:
metallophosphoesterase domain containing 1
Transcript:
RefSeq NM_001585.2 (NON-CURRENT)
Match location:
Position 568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTCATCAAGCAGGACTTT
Hairpin Sequence:
5'-CCGG-GACCTCATCAAGCAGGACTTT-CTCGAG-AAAGTCCTGCTTGATGAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTCATCAAGCAGGACTTTCTCGAGAAAGTCCTGCTTGATGAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTCATCAAGCAGGACTTTCTCGAGAAAGTCCTGCTTGATGAGGTC-3'

Other clones with same target sequence:

  1. TRCN0000182734