Construct: shRNA TRCN0000050560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371214.2-3339s1c1
DNA Barcode:
GCCCGTGATTAGAAGGGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLCH2 (9651)
Gene Description:
phospholipase C eta 2
Transcript:
RefSeq XM_371214.2 (NON-CURRENT)
Match location:
Position 3339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGTGATTAGAAGGGTGAA
Hairpin Sequence:
5'-CCGG-GCCCGTGATTAGAAGGGTGAA-CTCGAG-TTCACCCTTCTAATCACGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGTGATTAGAAGGGTGAACTCGAGTTCACCCTTCTAATCACGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGTGATTAGAAGGGTGAACTCGAGTTCACCCTTCTAATCACGGGC-3'

Other clones with same target sequence:

(none)