Construct: shRNA TRCN0000050586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006703.2-799s1c1
DNA Barcode:
GCTCGATGTCAGGCATCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDT3 (11165)
Gene Description:
nudix hydrolase 3
Transcript:
RefSeq NM_006703.2 (NON-CURRENT)
Match location:
Position 799 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGATGTCAGGCATCAGAT
Hairpin Sequence:
5'-CCGG-GCTCGATGTCAGGCATCAGAT-CTCGAG-ATCTGATGCCTGACATCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGATGTCAGGCATCAGATCTCGAGATCTGATGCCTGACATCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGATGTCAGGCATCAGATCTCGAGATCTGATGCCTGACATCGAGC-3'

Other clones with same target sequence:

  1. TRCN0000331660