Construct: shRNA TRCN0000050614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015455.2-823s1c1
DNA Barcode:
CCAGGATATATTGAACCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNOT6 (57472)
Gene Description:
CCR4-NOT transcription complex subunit 6
Transcript:
RefSeq NM_015455.2 (NON-CURRENT)
Match location:
Position 823 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGATATATTGAACCTTTA
Hairpin Sequence:
5'-CCGG-CCAGGATATATTGAACCTTTA-CTCGAG-TAAAGGTTCAATATATCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGATATATTGAACCTTTACTCGAGTAAAGGTTCAATATATCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGATATATTGAACCTTTACTCGAGTAAAGGTTCAATATATCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000299414