Construct: shRNA TRCN0000050654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182503.1-888s1c1
DNA Barcode:
TGGGAGACCATTTCAGTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAT2 (134637)
Gene Description:
adenosine deaminase tRNA specific 2
Transcript:
RefSeq NM_182503.1 (NON-CURRENT)
Match location:
Position 888 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGAGACCATTTCAGTGTAT
Hairpin Sequence:
5'-CCGG-TGGGAGACCATTTCAGTGTAT-CTCGAG-ATACACTGAAATGGTCTCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGAGACCATTTCAGTGTATCTCGAGATACACTGAAATGGTCTCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGAGACCATTTCAGTGTATCTCGAGATACACTGAAATGGTCTCCCA-3'

Other clones with same target sequence:

(none)