Construct: shRNA TRCN0000050663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018183.2-3516s1c1
DNA Barcode:
CCACGTAGAATTATACACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SBNO1 (55206)
Gene Description:
strawberry notch homolog 1
Transcript:
RefSeq NM_018183.2 (NON-CURRENT)
Match location:
Position 3516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGTAGAATTATACACAAT
Hairpin Sequence:
5'-CCGG-CCACGTAGAATTATACACAAT-CTCGAG-ATTGTGTATAATTCTACGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGTAGAATTATACACAATCTCGAGATTGTGTATAATTCTACGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGTAGAATTATACACAATCTCGAGATTGTGTATAATTCTACGTGG-3'

Other clones with same target sequence:

(none)