Construct: shRNA TRCN0000050685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_294540.3-758s1c1
DNA Barcode:
GCTGCTGTCATCCTTCAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACER2 (340485)
Gene Description:
alkaline ceramidase 2
Transcript:
RefSeq XM_294540.3 (NON-CURRENT)
Match location:
Position 758 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGTCATCCTTCAACTT
Hairpin Sequence:
5'-CCGG-GCTGCTGTCATCCTTCAACTT-CTCGAG-AAGTTGAAGGATGACAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGTCATCCTTCAACTTCTCGAGAAGTTGAAGGATGACAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGTCATCCTTCAACTTCTCGAGAAGTTGAAGGATGACAGCAGC-3'

Other clones with same target sequence:

(none)