Construct: shRNA TRCN0000050725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004352.1-633s1c1
DNA Barcode:
CCAAGTGCAAAGCCATCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLJ16323 (441390)
Gene Description:
FLJ16323 protein
Transcript:
RefSeq NM_001004352.1 (NON-CURRENT)
Match location:
Position 633 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTGCAAAGCCATCTCAA
Hairpin Sequence:
5'-CCGG-CCAAGTGCAAAGCCATCTCAA-CTCGAG-TTGAGATGGCTTTGCACTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTGCAAAGCCATCTCAACTCGAGTTGAGATGGCTTTGCACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTGCAAAGCCATCTCAACTCGAGTTGAGATGGCTTTGCACTTGG-3'

Other clones with same target sequence:

(none)