Construct: shRNA TRCN0000050737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_030577.8-1212s1c1
DNA Barcode:
CCTTTCACCTATGAAAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP9A (10079)
Gene Description:
ATPase phospholipid transporting 9A (putative)
Transcript:
RefSeq XM_030577.8 (NON-CURRENT)
Match location:
Position 1212 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCACCTATGAAAGCAAA
Hairpin Sequence:
5'-CCGG-CCTTTCACCTATGAAAGCAAA-CTCGAG-TTTGCTTTCATAGGTGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCACCTATGAAAGCAAACTCGAGTTTGCTTTCATAGGTGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCACCTATGAAAGCAAACTCGAGTTTGCTTTCATAGGTGAAAGG-3'

Other clones with same target sequence:

(none)