Construct: shRNA TRCN0000050805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025003.2-2097s1c1
DNA Barcode:
CGTGTTAAACTCCAGTGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAMTS20 (80070)
Gene Description:
ADAM metallopeptidase with thrombospondin type 1 motif 20
Transcript:
RefSeq NM_025003.2 (NON-CURRENT)
Match location:
Position 2097 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTTAAACTCCAGTGCCAA
Hairpin Sequence:
5'-CCGG-CGTGTTAAACTCCAGTGCCAA-CTCGAG-TTGGCACTGGAGTTTAACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTTAAACTCCAGTGCCAACTCGAGTTGGCACTGGAGTTTAACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTTAAACTCCAGTGCCAACTCGAGTTGGCACTGGAGTTTAACACG-3'

Other clones with same target sequence:

(none)