Construct: shRNA TRCN0000050843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_496393.1-2839s1c1
DNA Barcode:
CCACAAATTGACACTTTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDT4B (440672)
Gene Description:
nudix hydrolase 4B
Transcript:
RefSeq XM_496393.1 (NON-CURRENT)
Match location:
Position 2839 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAAATTGACACTTTCTAT
Hairpin Sequence:
5'-CCGG-CCACAAATTGACACTTTCTAT-CTCGAG-ATAGAAAGTGTCAATTTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAAATTGACACTTTCTATCTCGAGATAGAAAGTGTCAATTTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAAATTGACACTTTCTATCTCGAGATAGAAAGTGTCAATTTGTGG-3'

Other clones with same target sequence:

(none)