Construct: shRNA TRCN0000050863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000895.1-1773s1c1
DNA Barcode:
CGGCCCTTATTCAAGGATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LTA4H (4048)
Gene Description:
leukotriene A4 hydrolase
Transcript:
RefSeq NM_000895.1 (NON-CURRENT)
Match location:
Position 1773 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCCTTATTCAAGGATCTT
Hairpin Sequence:
5'-CCGG-CGGCCCTTATTCAAGGATCTT-CTCGAG-AAGATCCTTGAATAAGGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCCTTATTCAAGGATCTTCTCGAGAAGATCCTTGAATAAGGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCCTTATTCAAGGATCTTCTCGAGAAGATCCTTGAATAAGGGCCG-3'

Other clones with same target sequence:

  1. TRCN0000298973