Construct: shRNA TRCN0000050871
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001775.2-979s1c1
- DNA Barcode:
- CTGAGGATTCATCTTGCACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CD38 (952)
- Gene Description:
- CD38 molecule
- Transcript:
- RefSeq NM_001775.2 (NON-CURRENT)
- Match location:
- Position 979 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000050871)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGAGGATTCATCTTGCACAT
- Hairpin Sequence:
-
5'-CCGG-CTGAGGATTCATCTTGCACAT-CTCGAG-ATGTGCAAGATGAATCCTCAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGAGGATTCATCTTGCACATCTCGAGATGTGCAAGATGAATCCTCAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGAGGATTCATCTTGCACATCTCGAGATGTGCAAGATGAATCCTCAG-3'
Other clones with same target sequence:
(none)
