Construct: shRNA TRCN0000050878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005033.1-1260s1c1
DNA Barcode:
GCTATCATTCTTGATGGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOSC9 (5393)
Gene Description:
exosome component 9
Transcript:
RefSeq NM_005033.1 (NON-CURRENT)
Match location:
Position 1260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATCATTCTTGATGGTATA
Hairpin Sequence:
5'-CCGG-GCTATCATTCTTGATGGTATA-CTCGAG-TATACCATCAAGAATGATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATCATTCTTGATGGTATACTCGAGTATACCATCAAGAATGATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATCATTCTTGATGGTATACTCGAGTATACCATCAAGAATGATAGC-3'

Other clones with same target sequence:

  1. TRCN0000327853