Construct: shRNA TRCN0000050901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370652.2-3432s1c1
DNA Barcode:
CCTGGCAAGTAGTATCTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYNC2H1 (79659)
Gene Description:
dynein cytoplasmic 2 heavy chain 1
Transcript:
RefSeq XM_370652.2 (NON-CURRENT)
Match location:
Position 3432 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCAAGTAGTATCTCTAA
Hairpin Sequence:
5'-CCGG-CCTGGCAAGTAGTATCTCTAA-CTCGAG-TTAGAGATACTACTTGCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCAAGTAGTATCTCTAACTCGAGTTAGAGATACTACTTGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCAAGTAGTATCTCTAACTCGAGTTAGAGATACTACTTGCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000288797