Construct: shRNA TRCN0000050952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012229.2-671s1c1
DNA Barcode:
CCAGATATACCAGTTGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NT5C2 (22978)
Gene Description:
5'-nucleotidase, cytosolic II
Transcript:
RefSeq NM_012229.2 (NON-CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATATACCAGTTGTGAAA
Hairpin Sequence:
5'-CCGG-CCAGATATACCAGTTGTGAAA-CTCGAG-TTTCACAACTGGTATATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATATACCAGTTGTGAAACTCGAGTTTCACAACTGGTATATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATATACCAGTTGTGAAACTCGAGTTTCACAACTGGTATATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000289475