Construct: shRNA TRCN0000050962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006290.2-2104s1c1
DNA Barcode:
GAAGCTCAGAATCAGAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFAIP3 (7128)
Gene Description:
TNF alpha induced protein 3
Transcript:
RefSeq NM_006290.2 (NON-CURRENT)
Match location:
Position 2104 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000050962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTCAGAATCAGAGATTT
Hairpin Sequence:
5'-CCGG-GAAGCTCAGAATCAGAGATTT-CTCGAG-AAATCTCTGATTCTGAGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTCAGAATCAGAGATTTCTCGAGAAATCTCTGATTCTGAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTCAGAATCAGAGATTTCTCGAGAAATCTCTGATTCTGAGCTTC-3'

Other clones with same target sequence:

(none)