Construct: shRNA TRCN0000051082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370754.2-419s1c1
DNA Barcode:
GAACCTACAATTGTGGCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THTPA (79178)
Gene Description:
thiamine triphosphatase
Transcript:
RefSeq XM_370754.2 (NON-CURRENT)
Match location:
Position 419 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTACAATTGTGGCCCAA
Hairpin Sequence:
5'-CCGG-GAACCTACAATTGTGGCCCAA-CTCGAG-TTGGGCCACAATTGTAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTACAATTGTGGCCCAACTCGAGTTGGGCCACAATTGTAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTACAATTGTGGCCCAACTCGAGTTGGGCCACAATTGTAGGTTC-3'

Other clones with same target sequence:

(none)