Construct: shRNA TRCN0000051083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024490.2-931s1c1
DNA Barcode:
CGGCTGCATCATACATGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP10A (57194)
Gene Description:
ATPase phospholipid transporting 10A (putative)
Transcript:
RefSeq NM_024490.2 (NON-CURRENT)
Match location:
Position 931 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTGCATCATACATGACAA
Hairpin Sequence:
5'-CCGG-CGGCTGCATCATACATGACAA-CTCGAG-TTGTCATGTATGATGCAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTGCATCATACATGACAACTCGAGTTGTCATGTATGATGCAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTGCATCATACATGACAACTCGAGTTGTCATGTATGATGCAGCCG-3'

Other clones with same target sequence:

(none)