Construct: shRNA TRCN0000051136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014905.2-1576s1c1
DNA Barcode:
GCCCTGAAGCAGTTCGAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLS (2744)
Gene Description:
glutaminase
Transcript:
RefSeq NM_014905.2 (NON-CURRENT)
Match location:
Position 1576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGAAGCAGTTCGAAATA
Hairpin Sequence:
5'-CCGG-GCCCTGAAGCAGTTCGAAATA-CTCGAG-TATTTCGAACTGCTTCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGAAGCAGTTCGAAATACTCGAGTATTTCGAACTGCTTCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGAAGCAGTTCGAAATACTCGAGTATTTCGAACTGCTTCAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000299000