Construct: shRNA TRCN0000051159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138448.2-541s1c1
DNA Barcode:
TGGGTGAAGAATACCAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACYP2 (98)
Gene Description:
acylphosphatase 2
Transcript:
RefSeq NM_138448.2 (NON-CURRENT)
Match location:
Position 541 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGTGAAGAATACCAGCAAA
Hairpin Sequence:
5'-CCGG-TGGGTGAAGAATACCAGCAAA-CTCGAG-TTTGCTGGTATTCTTCACCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGTGAAGAATACCAGCAAACTCGAGTTTGCTGGTATTCTTCACCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGTGAAGAATACCAGCAAACTCGAGTTTGCTGGTATTCTTCACCCA-3'

Other clones with same target sequence:

  1. TRCN0000299074