Construct: shRNA TRCN0000051206

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012080.1-138s1c1
DNA Barcode:
GTTATGGGTAAGAAGGCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PUDP (8226)
Gene Description:
pseudouridine 5'-phosphatase
Transcript:
RefSeq NM_012080.1 (NON-CURRENT)
Match location:
Position 138 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051206)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTATGGGTAAGAAGGCATTA
Hairpin Sequence:
5'-CCGG-GTTATGGGTAAGAAGGCATTA-CTCGAG-TAATGCCTTCTTACCCATAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTATGGGTAAGAAGGCATTACTCGAGTAATGCCTTCTTACCCATAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTATGGGTAAGAAGGCATTACTCGAGTAATGCCTTCTTACCCATAAC-3'

Other clones with same target sequence:

(none)