Construct: shRNA TRCN0000051344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014960.2-1383s1c1
DNA Barcode:
GCAGCATAAGTTTCCTCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARSG (22901)
Gene Description:
arylsulfatase G
Transcript:
RefSeq NM_014960.2 (NON-CURRENT)
Match location:
Position 1383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCATAAGTTTCCTCTGAT
Hairpin Sequence:
5'-CCGG-GCAGCATAAGTTTCCTCTGAT-CTCGAG-ATCAGAGGAAACTTATGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCATAAGTTTCCTCTGATCTCGAGATCAGAGGAAACTTATGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCATAAGTTTCCTCTGATCTCGAGATCAGAGGAAACTTATGCTGC-3'

Other clones with same target sequence:

(none)