Construct: shRNA TRCN0000051393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003291.1-3128s1c1
DNA Barcode:
GCTGGATTCTAGTGACATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPP2 (7174)
Gene Description:
tripeptidyl peptidase 2
Transcript:
RefSeq NM_003291.1 (NON-CURRENT)
Match location:
Position 3128 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATTCTAGTGACATTTA
Hairpin Sequence:
5'-CCGG-GCTGGATTCTAGTGACATTTA-CTCGAG-TAAATGTCACTAGAATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATTCTAGTGACATTTACTCGAGTAAATGTCACTAGAATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATTCTAGTGACATTTACTCGAGTAAATGTCACTAGAATCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000299615