Construct: shRNA TRCN0000051459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020162.2-660s1c1
DNA Barcode:
CTCGGGAAACTTCCTCTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHX33 (56919)
Gene Description:
DEAH-box helicase 33
Transcript:
RefSeq NM_020162.2 (NON-CURRENT)
Match location:
Position 660 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGGGAAACTTCCTCTGAAA
Hairpin Sequence:
5'-CCGG-CTCGGGAAACTTCCTCTGAAA-CTCGAG-TTTCAGAGGAAGTTTCCCGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGGGAAACTTCCTCTGAAACTCGAGTTTCAGAGGAAGTTTCCCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGGGAAACTTCCTCTGAAACTCGAGTTTCAGAGGAAGTTTCCCGAG-3'

Other clones with same target sequence:

(none)