Construct: shRNA TRCN0000051494

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000439.3-2436s1c1
DNA Barcode:
GCTCTGGTGGACATTCTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCSK1 (5122)
Gene Description:
proprotein convertase subtilisin/kexin type 1
Transcript:
RefSeq NM_000439.3 (NON-CURRENT)
Match location:
Position 2436 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051494)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGGTGGACATTCTGAAT
Hairpin Sequence:
5'-CCGG-GCTCTGGTGGACATTCTGAAT-CTCGAG-ATTCAGAATGTCCACCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGGTGGACATTCTGAATCTCGAGATTCAGAATGTCCACCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGGTGGACATTCTGAATCTCGAGATTCAGAATGTCCACCAGAGC-3'

Other clones with same target sequence:

(none)