Construct: shRNA TRCN0000051597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024302.2-387s1c1
DNA Barcode:
GCATTCCTAGAGAAGTACGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MMP28 (79148)
Gene Description:
matrix metallopeptidase 28
Transcript:
RefSeq NM_024302.2 (NON-CURRENT)
Match location:
Position 387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCCTAGAGAAGTACGGA
Hairpin Sequence:
5'-CCGG-GCATTCCTAGAGAAGTACGGA-CTCGAG-TCCGTACTTCTCTAGGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCCTAGAGAAGTACGGACTCGAGTCCGTACTTCTCTAGGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCCTAGAGAAGTACGGACTCGAGTCCGTACTTCTCTAGGAATGC-3'

Other clones with same target sequence:

(none)