Construct: shRNA TRCN0000051632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018199.1-1001s1c1
DNA Barcode:
CGGAAGAACGTGATTCCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXD2 (55218)
Gene Description:
exonuclease 3'-5' domain containing 2
Transcript:
RefSeq NM_018199.1 (NON-CURRENT)
Match location:
Position 1001 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGAACGTGATTCCACAT
Hairpin Sequence:
5'-CCGG-CGGAAGAACGTGATTCCACAT-CTCGAG-ATGTGGAATCACGTTCTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGAACGTGATTCCACATCTCGAGATGTGGAATCACGTTCTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGAACGTGATTCCACATCTCGAGATGTGGAATCACGTTCTTCCG-3'

Other clones with same target sequence:

  1. TRCN0000327822