Construct: shRNA TRCN0000051659

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004334.1-849s1c1
DNA Barcode:
GCAGCATGAAAGTCCTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BST1 (683)
Gene Description:
bone marrow stromal cell antigen 1
Transcript:
RefSeq NM_004334.1 (NON-CURRENT)
Match location:
Position 849 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051659)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCATGAAAGTCCTGGAAA
Hairpin Sequence:
5'-CCGG-GCAGCATGAAAGTCCTGGAAA-CTCGAG-TTTCCAGGACTTTCATGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCATGAAAGTCCTGGAAACTCGAGTTTCCAGGACTTTCATGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCATGAAAGTCCTGGAAACTCGAGTTTCCAGGACTTTCATGCTGC-3'

Other clones with same target sequence:

(none)