Construct: shRNA TRCN0000051674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005540.1-863s1c1
DNA Barcode:
GCGGGAACATACAATGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INPP5B (3633)
Gene Description:
inositol polyphosphate-5-phosphatase B
Transcript:
RefSeq NM_005540.1 (NON-CURRENT)
Match location:
Position 863 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGGAACATACAATGTAAAT
Hairpin Sequence:
5'-CCGG-GCGGGAACATACAATGTAAAT-CTCGAG-ATTTACATTGTATGTTCCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGGAACATACAATGTAAATCTCGAGATTTACATTGTATGTTCCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGGAACATACAATGTAAATCTCGAGATTTACATTGTATGTTCCCGC-3'

Other clones with same target sequence:

(none)